ERAP1 Recombinant Rabbit Monoclonal Antibody [JE52-36] (ET7110-29)
Catalog# ET7110-29
Rabbit Monoclonal to ERAP1
Tested for
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WB
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IHC-P
Reacts with
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Human
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
All lanes: Western blot analysis of ERAP1 with anti-ERAP1 antibody [JE52-36] (ET7110-29) at 1/500 dilution.
Lane 1: Wild-type 293T whole cell lysate.
Lane 2: ERAP1 knockout 293T whole cell lysate.
ET7110-29 was shown to specifically react with ERAP1 in wild-type 293T cells. No band was observed when ERAP1 knockout sample was tested. Wild-type and ERAP1 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-ERAP1 antibody (ET7110-29, 1/500) and Anti-β-actin antibody (R1207-1, 1/1,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China).
Lane 1: Wild-type 293T whole cell lysate.
Lane 2: ERAP1 knockout 293T whole cell lysate.
ET7110-29 was shown to specifically react with ERAP1 in wild-type 293T cells. No band was observed when ERAP1 knockout sample was tested. Wild-type and ERAP1 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-ERAP1 antibody (ET7110-29, 1/500) and Anti-β-actin antibody (R1207-1, 1/1,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China).