Neuropilin 1 Recombinant Rabbit Monoclonal Antibody [ST05-30] (ET1609-69)
Catalog# ET1609-69
Rabbit Monoclonal to Neuropilin 1
Tested for
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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IP
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
This product has been cited in peer reviewed publications, see list HERE
Western blot analysis of Neuropilin 1 on different lysates with Rabbit anti-Neuropilin 1 antibody (ET1609-69) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.
Lane 1: U-87 MG cell lysate
Lane 2: A549 cell lysate
Lane 3: SK-Br-3 cell lysate (low expression)
Lane 4: MDA-MB-231 cell lysate
Lane 5: Mouse brain tissue lysate
Lane 6: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 103 kDa
Observed band size: 130 kDa
Exposure time: 35 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-69) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: U-87 MG cell lysate
Lane 2: A549 cell lysate
Lane 3: SK-Br-3 cell lysate (low expression)
Lane 4: MDA-MB-231 cell lysate
Lane 5: Mouse brain tissue lysate
Lane 6: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 103 kDa
Observed band size: 130 kDa
Exposure time: 35 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-69) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
