CD99 Recombinant Rabbit Monoclonal Antibody [JF0991] (ET1702-35)
Catalog# ET1702-35
Rabbit Monoclonal to CD99
Tested for
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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IP
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FC
Reacts with
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Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
This product has been cited in peer reviewed publications, see list HERE
Western blot analysis of CD99 on different lysates with Rabbit anti-CD99 antibody (ET1702-35) at 1/1,000 dilution.
Lane 1: THP-1 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: U-937 cell lysate
Lane 4: MOLT-4 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 19 kDa
Observed band size: 30 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-35) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: THP-1 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: U-937 cell lysate
Lane 4: MOLT-4 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 19 kDa
Observed band size: 30 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-35) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
