DKK1 Recombinant Rabbit Monoclonal Antibody [SC06-86] (ET1610-63)
Catalog# ET1610-63
Rabbit Monoclonal to DKK1
Tested for
-
WB
-
IF-Cell
-
IF-Tissue
-
IHC-P
-
FC
Reacts with
-
Human
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
This product has been cited in peer reviewed publications, see list HERE
Western blot analysis of DKK1 on Caco-2 cell lysates with Rabbit anti-DKK1 antibody (ET1610-63) at 1/2,000 dilution.
Lysates/proteins at 15 µg/Lane.
Predicted band size: 29 kDa
Observed band size: 35-40 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-63) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lysates/proteins at 15 µg/Lane.
Predicted band size: 29 kDa
Observed band size: 35-40 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-63) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
