Nectin 2 Recombinant Rabbit Monoclonal Antibody [PSH11-54] (HA723346)
Catalog# HA723346
Rabbit Monoclonal to Nectin 2
Tested for
-
WB
-
IF-Cell
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FC
Reacts with
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Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Nectin 2 on different lysates with Rabbit anti-Nectin 2 antibody (HA723346) at 1/2,000 dilution.
Lane 1: MCF7 cell lysate
Lane 2: MOLT-4 cell lysate (negative)
Lane 3: HT-29 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 58 kDa
Observed band size: 58-75 kDa
Exposure time: 25 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723346) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: MCF7 cell lysate
Lane 2: MOLT-4 cell lysate (negative)
Lane 3: HT-29 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 58 kDa
Observed band size: 58-75 kDa
Exposure time: 25 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723346) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.