TdT Recombinant Rabbit Monoclonal Antibody [PD01-00] (HA721211)
Catalog# HA721211
Rabbit Monoclonal to TdT
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of TdT on different lysates with Rabbit anti-TdT antibody (HA721211) at 1/1,000 dilution.
Lane 1: Jurkat cell lysate (20 µg/Lane)
Lane 2: A431 cell lysate (negative) (20 µg/Lane)
Lane 3: Raji cell lysate (negative) (20 µg/Lane)
Lane 4: Daudi cell lysate (negative) (20 µg/Lane)
Lane 5: MOLT-4 cell lysate (20 µg/Lane)
Predicted band size: 59 kDa
Observed band size: 55 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721211) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Jurkat cell lysate (20 µg/Lane)
Lane 2: A431 cell lysate (negative) (20 µg/Lane)
Lane 3: Raji cell lysate (negative) (20 µg/Lane)
Lane 4: Daudi cell lysate (negative) (20 µg/Lane)
Lane 5: MOLT-4 cell lysate (20 µg/Lane)
Predicted band size: 59 kDa
Observed band size: 55 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721211) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.