CXCR4 Recombinant Rabbit Monoclonal Antibody [JE03-35] (HA722304)
Catalog# HA722304
Rabbit Monoclonal to CXCR4
Tested for
-
WB
-
IF-Cell
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IHC-P
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
This product has been cited in peer reviewed publications, see list HERE
Western blot analysis of CXCR4 on different lysates with Rabbit anti-CXCR4 antibody (HA722304) at 1/1,000 dilution.
Lane 1: Jurkat cell lysate (hot lysis)
Lane 2: HeLa cell lysate (hot lysis)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 40 kDa
Observed band size: 45 kDa
Exposure time: 25 second; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722304) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Jurkat cell lysate (hot lysis)
Lane 2: HeLa cell lysate (hot lysis)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 40 kDa
Observed band size: 45 kDa
Exposure time: 25 second; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722304) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
