B7-H6 Recombinant Rabbit Monoclonal Antibody [PSH10-89] (HA723256)
Catalog# HA723256
Rabbit Monoclonal to B7-H6
Tested for
-
WB
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IF-Cell
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FC
-
IP
Reacts with
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Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of B7-H6 on different lysates with Rabbit anti-B7-H6 antibody (HA723256) at 1/2,000 dilution.
Lane 1: K-562 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: MCF7 cell lysate (negative)
Lane 4: HeLa cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 51 kDa
Observed band size: 75 kDa
Exposure time: 2 minutes 41 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723256) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: K-562 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: MCF7 cell lysate (negative)
Lane 4: HeLa cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 51 kDa
Observed band size: 75 kDa
Exposure time: 2 minutes 41 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723256) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.