CCR7 Recombinant Rabbit Monoclonal Antibody [SR36-04] (ET1602-22)
Catalog# ET1602-22
Rabbit Monoclonal to CCR7
Tested for
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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IP
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
This product has been cited in peer reviewed publications, see list HERE
All lanes: Western blot analysis of CCR7 with anti-CCR7 antibody (ET1602-22) at 1:5,000 dilution.
Lane 1/2: Wild-type Hela whole cell lysate (10 µg).
Lane 3/4: CCR7 fragment 1 knockdown Hela whole cell lysate (10 µg).
Lane 5/6: CCR7 fragment 2 knockdown Hela whole cell lysate (10 µg).
ET1602-22 was shown to specifically react with CCR7 in wild-type Hela cells. Weakened was observed when CCR7 knockdown sample was tested. Wild-type and CCR7 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1602-22, 1/500) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Lane 1/2: Wild-type Hela whole cell lysate (10 µg).
Lane 3/4: CCR7 fragment 1 knockdown Hela whole cell lysate (10 µg).
Lane 5/6: CCR7 fragment 2 knockdown Hela whole cell lysate (10 µg).
ET1602-22 was shown to specifically react with CCR7 in wild-type Hela cells. Weakened was observed when CCR7 knockdown sample was tested. Wild-type and CCR7 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1602-22, 1/500) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
