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HRP Conjugated beta Actin Recombinant Rabbit Monoclonal Antibody [JF53-10] (ET1702-67)

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Catalog# ET1702-67

Rabbit Monoclonal to beta Actin

Tested for

  • WB

Reacts with

  • Human

  • Mouse

  • Rat

  • Zebrafish

  • Monkey

  • Hamster

  • Arabidopsis thaliana

  • Rice

Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Conjugated to HRP
This product has been cited in peer reviewed publications, see list HERE

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Specifications

Product Type

Recombinant Rabbit monoclonal primary

Product Name

HRP Conjugated beta Actin Recombinant Rabbit Monoclonal Antibody [JF53-10] (ET1702-67)

Immunogen

Synthetic peptide within n-terminal residues of β-actin.

Host

Rabbit

Positive Control

HeLa cell lysate, HepG2 cell lysate, MCF7 cell lysate, A431 cell lysate, Jurkat cell lysate, HEK-293 cell lysate, RAW264.7 cell lysate, C2C12 cell lysate, PC-12 cell lysate, Mouse testis tissue lysate, Mouse spleen tissue lysate, Rat testis tissue lysate, Rat spleen tissue lysate.

Conjugation

Hrp

Clonality

Monoclonal

Clone Number

JF53-10

RRID

AB_2890197

APPLICATION DILUTION

  • WB

  • 1:20,000-1:50,000

PROPERTIES

Form

Liquid

Storage Condition

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol.

Concentration

1ug/ul

Purification

Protein A affinity purified.

Molecular Weight

Predicted band size: 42 kDa

Isotype

IgG

TARGET

UNIPROT #

P60709

PROTEIN NAME

beta Actin

SYNONYMS

A26C1A antibody;A26C1B antibody;ACTB antibody;ACTB_HUMAN antibody;Actin beta antibody;Actin cytoplasmic 1 antibody;Actin, cytoplasmic 1, N-terminally processed antibody;Actx antibody;b actin antibody;Beta cytoskeletal actin antibody;Beta-actin antibody;BRWS1 antibody;E430023M04Rik antibody;MGC128179 antibody;PS1TP5 binding protein 1 antibody;PS1TP5BP1 antibody;β-actin antibody;β actin antibody

SEQUENCE SIMILARITIES

Belongs to the actin family.

POST-TRANSLATIONAL MODIFICATION

ISGylated.; Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization.; Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.; Methylated at His-73 by SETD3. Methylation at His-73 is required for smooth muscle contraction of the laboring uterus during delivery (By similarity).; [Actin, cytoplasmic 1, N-terminally processed]: N-terminal acetylation by NAA80 affects actin filament depolymerization and elongation, including elongation driven by formins. In contrast, filament nucleation by the Arp2/3 complex is not affected.; (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding. The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. α-Actin expression is limited to various types of muscle, whereas β-Actin and γ-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia.

CITATIONS

  • Wu, A., Sun, W., & Mou, F.

    lncRNA‑MALAT1 promotes high glucose‑induced H9C2 cardiomyocyte pyroptosis by downregulating miR‑141‑3p expression. Molecular medicine reports, 23(4), 259.

  • Wang, Z. Q., & Sun, Z.

    Dietary Nε-(carboxymethyl) lysine affects cardiac glucose metabolism and myocardial remodeling in mice

  • Xiaoxiao Li,et al

    Cholesterol overload in macrophages drives metabolic dysfunction-associated steatohepatitis via inhibiting 7-dehydrocholesterol reductase in mice

  • Long, Q., Yan, K., Wang, C., Wen, Y., Qi, F., Wang, H., Shi, P., Liu, X., Chan, W. Y., Lu, X., & Zhao, H.

    Modification of maternally defined H3K4me3 regulates the inviability of interspecific Xenopus hybrids

  • Jiang SJ, Xiao X, Li J, Mu Y

    Lycium barbarum polysaccharide-glycoprotein ameliorates ionizing radiation-induced epithelial injury by regulating oxidative stress and ferroptosis via the Nrf2 pathway

  • Voluven treatment could induce NLRP3 inflammasome-mediated pyroptosis of bone marrow-derived macrophages

  • Keliang Xie

    Protective effects of hydrogen gas against sepsis-induced acute lung injury via regulation of mitochondrial function and dynamics.

  • Guang Ji

    Comprehensive analysis of 5‐hydroxymethylcytosine in zw10 kinetochore protein as a promising biomarker for screening and diagnosis of early colorectal cancer

  • Wang Zhuang,et al

    Tissue-resident trained immunity in hepatocytes protects against septic liver injury in zebrafish

  • Yang Liang,et al

    NAD+ dependent UPRmt activation underlies intestinal aging caused by mitochondrial DNA mutations

  • Li Wen,et al

    Mass Spectrometry-Based Proteomic Analysis of Potential Host Proteins Interacting with GP5 in PRRSV-Infected PAMs

  • Yafei Wu

    Fusobacterium nucleatum stimulates monocyte adhesion to and transmigration through endothelial cells

  • Chen Jinyong,et al

    Enhancing aortic valve drug delivery with PAR2-targeting magnetic nano-cargoes for calcification alleviation

  • Qin Beibei,et al

    TRIM103 activates the RLRs pathway to enhance antiviral response by targeting VP5 and VP7

  • HuZhijuan,et al

    A novel protein CYTB-187AA encoded by the mitochondrial gene CYTB modulates mammalian early development

  • Wang Chenlu,et al

    Genome-wide CRISPR screenings identified SMCHD1 as a host-restricting factor for AAV transduction

  • Liu Li,et al

    Ghrelin suppresses hypoxia/reoxygenation-induced H9C2 cell pyroptosis via NLRP3

  • Xie Feiyan,et al

    ZBP1 condensate formation synergizes Z-NAs recognition and signal transduction

  • Guang Ji

    Ling-gui-zhu-gan decoction alleviates hepatic steatosis through SOCS2 modification by N6-methyladenosine

  • Geng Xiaoyu,et al

    Comparative Analysis of Tentacle Extract and Nematocyst Venom: Toxicity, Mechanism, and Potential Intervention in the Giant Jellyfish Nemopilema nomurai

  • Liu Liang,et al

    Tissue-resident C1q+ macrophages exert anti-aging potential through the Sirt1 pathway

  • Han Sun,et al

    Isoechinulin B, a natural product from Antarctic fungus, attenuates acute liver injury by inhibiting excessive cell adhesion

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