ADFP / PLIN2 Recombinant Rabbit Monoclonal Antibody [JA31-81] (ET1704-17)
Catalog# ET1704-17
Rabbit Monoclonal to ADFP
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WB
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IF-Cell
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IF-Tissue
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FC
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IHC-P
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Human
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Mouse
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Rat
Lane 1: HepG2 cell lysate
Lane 2: JAR cell lysate
Lane 3: Human liver tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 48 kDa
Observed band size: 45 kDa
Exposure time: 1 minute 2 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-17) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
ADFP / PLIN2 Recombinant Rabbit Monoclonal Antibody [JA31-81] (ET1704-17)
Immunogen
Synthetic peptide within human adfp aa 18-67 / 437.
Host
Rabbit
Positive Control
HepG2 cell lysate, JAR cell lysate, Human liver tissue lysate, HeLa, human liver tissue, human liver carcinoma tissue, human stomach tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JA31-81
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IF-Cell
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1:50-1:200
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IF-Tissue
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1:50-1:200
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FC
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1:1,000
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IHC-P
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1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 48 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
ADFP
SYNONYMS
ADFP antibody;Adipophilin antibody;Adipose differentiation related protein antibody;Adipose differentiation-related protein antibody;ADRP antibody;MGC10598 antibody;Perilipin-2 antibody;PLIN2 antibody;PLIN2_HUMAN antibody
SEQUENCE SIMILARITIES
Belongs to the perilipin family.
TISSUE SPECIFICITY
Milk lipid globules.
POST-TRANSLATIONAL MODIFICATION
Acylated; primarily with C14, C16 and C18 fatty acids.
SUBCELLULAR LOCATION
Membrane.
FUNCTION
The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene.
CITATIONS
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Yang, G., Li, S., Zhao, Q., Chu, J., Zhou, B., Fan, S., Shi, F., Wei, X., Hu, X., Zheng, X., Liu, Z., Zhou, X., Tao, Y., Li, S., & Mou, C.
Transcriptomic and metabolomic insights into the variety of sperm storage in oviduct of egg layers. Poultry science, 100(6), 101087.
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IHC
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Human
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Citation
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