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MLKL Recombinant Rabbit Monoclonal Antibody [SA40-04] (ET1601-25)

Price: Regular price $385.00

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Catalog# ET1601-25

Rabbit Monoclonal to MLKL

Tested for

  • WB

  • IHC-P

  • IF-Cell

  • FC

Reacts with

  • Human

  • Mouse

Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
This product has been cited in peer reviewed publications, see list HERE

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Specifications

Product Type

Recombinant Rabbit monoclonal primary

Product Name

MLKL Recombinant Rabbit Monoclonal Antibody [SA40-04] (ET1601-25)

Immunogen

Recombinant protein within human mlkl aa 333-471 / 471.

Host

Rabbit

Positive Control

HUVEC cell lysate, HT-29 cell lysate, HeLa cell lysate, THP-1 cell lysate, U-937 cell lysate, K-562 cell lysate, NIH/3T3 cell lysate, HeLa, HT-29, human tonsil tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SA40-04

RRID

AB_2889917

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • IHC-P

  • 1:50-1:2000

  • IF-Cell

  • 1:100

  • FC

  • 1:1,000

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1ug/ul

Purification

Protein A affinity purified.

Molecular Weight

Predicted band size: 54 kDa

Isotype

IgG

TARGET

UNIPROT #

Q8NB16

PROTEIN NAME

MLKL

SYNONYMS

9130019I15Rik antibody;FLJ34389 antibody;hMLKL antibody;Mixed lineage kinase domain like antibody;Mixed lineage kinase domain like protein antibody;Mixed lineage kinase domain-like protein antibody;Mlkl antibody;MLKL_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation by RIPK3 induces a conformational switch that is required for necroptosis. It also induces homotrimerization and localization to the plasma membrane.

SUBCELLULAR LOCATION

Cytoplasm, Cell membrane, Golgi apparatus.

FUNCTION

Mixed lineage kinase domain like pseudokinase (MLKL) is a protein that in humans is encoded by the MLKL gene. This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis.

CITATIONS

  • He Ruobing,et al

    Aurantiamide mitigates acute kidney injury by suppressing renal necroptosis and inflammation via GRPR-dependent mechanism

  • Chen Ying,et al

    Cpd-A1 alleviates acute kidney injury by inhibiting ferroptosis

  • Dou, X., Yu, X., Du, S., Han, Y., Li, L., Zhang, H., Yao, Y., Du, Y., Wang, X., Li, J., Yang, T., Zhang, W., Yang, C., Ma, F., & He, S.

    Interferon-mediated repression of miR-324-5p potentiates necroptosis to facilitate antiviral defense

  • Qian, A., Xu, J., Wu, C., Liu, S., & Zhang, M.

    Hypothermia Inhibits Cerebral Necroptosis and NOD-Like Receptor Pyrin Domain Containing 3 Pathway in a Swine Model of Cardiac Arrest

  • Targeting Endothelial Necroptosis Disrupts Profibrotic Endothelial–Hepatic Stellate Cells Crosstalk to Alleviate Liver Fibrosis in Nonalcoholic Steatohepatitis

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