NF-L Rabbit Polyclonal Antibody (ER65439)
Catalog# ER65439
Rabbit Polyclonal to NF-L
Tested for
-
WB
-
IF-Cell
-
IHC-P
-
FC
Reacts with
-
Human
-
Mouse
-
Rat
Western blot analysis of NF-L on different lysates with Rabbit anti-NF-L antibody (ER65439) at 1/100,000 dilution.
Lane 1: Rat brain tissue lysate
Lane 2: Mouse hippocampus tissue lysate
Lane 3: Rat hippocampus tissue lysate
Lane 4: Human brain tissue lysate
Lysates/proteins at 5 µg/Lane.
Predicted band size: 62 kDa
Observed band size: 68 kDa
Exposure time: 3 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER65439) at 1/100,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Rat brain tissue lysate
Lane 2: Mouse hippocampus tissue lysate
Lane 3: Rat hippocampus tissue lysate
Lane 4: Human brain tissue lysate
Lysates/proteins at 5 µg/Lane.
Predicted band size: 62 kDa
Observed band size: 68 kDa
Exposure time: 3 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER65439) at 1/100,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.