Cathepsin B Mouse Monoclonal Antibody [J11-A11] (M1506-1)
Catalog# M1506-1
Mouse Monoclonal to Cathepsin B
Tested for
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WB
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IF-Cell
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IHC-P
Reacts with
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Human
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Zebrafish
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Mouse
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Rat
This product has been cited in peer reviewed publications, see list HERE
Western blot analysis of Cathepsin B on different lysates with Mouse anti-Cathepsin B antibody (M1506-1) at 1/1,000 dilution.
Lane 1: RAW264.7 cell lysate
Lane 2: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 38 kDa
Observed band size: 38 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1506-1) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: RAW264.7 cell lysate
Lane 2: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 38 kDa
Observed band size: 38 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1506-1) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
