Cathepsin B Recombinant Rabbit Monoclonal Antibody [JA11-02] (ET1704-03)
Catalog# ET1704-03
Rabbit Monoclonal to Cathepsin B
Tested for
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WB
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IF-Cell
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IF-Tissue
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IHC-P
Reacts with
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Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
This product has been cited in peer reviewed publications, see list HERE
Western blot analysis of Cathepsin B on different lysates with Rabbit anti-Cathepsin B antibody (ET1704-03) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: SW480 cell lysate
Lane 3: Daudi cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 38 kDa
Observed band size: 38 kDa
Exposure time: 1 minute 59 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-03) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HepG2 cell lysate
Lane 2: SW480 cell lysate
Lane 3: Daudi cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 38 kDa
Observed band size: 38 kDa
Exposure time: 1 minute 59 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-03) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
