Doublecortin Recombinant Rabbit Monoclonal Antibody [JJ0959] (ET1701-98)

UNIPROT #

O43602

PROTEIN NAME

Doublecortin

SYNONYMS

DBCN antibody;Dbct antibody;DC antibody;DCX antibody;DCX_HUMAN antibody;Doublecortex antibody;Doublin antibody;FLJ51296 antibody;Lis X antibody;Lis-X antibody;Lissencephalin X antibody;Lissencephalin-X antibody;Lissencephaly X linked antibody;Lissencephaly X linked doublecortin antibody;LISX antibody;Neuronal migration protein doublecortin antibody;OTTHUMP00000023859 antibody;OTTHUMP00000023860 antibody;OTTHUMP00000216315 antibody;OTTHUMP00000216316 antibody;SCLH antibody;XLIS antibody

TISSUE SPECIFICITY

Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation by MARK1, MARK2 and PKA regulates its ability to bind microtubules (By similarity). Phosphorylation at Ser-265 and Ser-297 seems to occur only in neonatal brain, the levels falling precipitously by postnatal day 21 (By similarity).; Ubiquitinated by MDM2, leading to its degradation by the proteasome. Ubiquitinated by MDM2 and subsequent degradation leads to reduce the dendritic spine density of olfactory bulb granule cells.

SUBCELLULAR LOCATION

Cytoplasm, Cell projection.

FUNCTION

Lissencephaly (smooth brain) is an abnormality of brain development characterized by incomplete neuronal migration and a smooth cerebral surface, resulting in severe mental retardation. Genetic analysis identified two proteins that are mutated in some cases of lissencephaly, designated lissencephaly-1 protein (LIS1) and doublecortin. LIS1 shows sequence homology to β-subunits of heterotrimeric G proteins. Doublecortin contains a consensus Abl phosphorylation site, and it has some sequence homology to a predicted kinase protein. Both proteins are highly expressed in developing brain, suggesting that they may be involved in a signal transduction pathway that is crucial to brain development.