DLK-1 Recombinant Rabbit Monoclonal Antibody [PSH04-76] (HA722180)
Catalog# HA722180
Rabbit Monoclonal to DLK-1
Tested for
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WB
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IHC-P
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IF-Tissue
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
This product has been cited in peer reviewed publications, see list HERE
Western blot analysis of DLK-1 on different lysates with Rabbit anti-DLK-1 antibody (HA722180) at 1/1,000 dilution.
Lane 1: MCF7 cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Lane 3: SH-SY5Y cell lysate (20 µg/Lane)
Lane 4: Mouse placenta tissue lysate (70℃ heat) (40 µg/Lane)
Lane 5: Rat embryo tissue lysate (40 µg/Lane)
Predicted band size: 41 kDa
Observed band size: 40-50kDa
Exposure time: Lane 1-4: 2 minutes; Lane 5: 27 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722180) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: MCF7 cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Lane 3: SH-SY5Y cell lysate (20 µg/Lane)
Lane 4: Mouse placenta tissue lysate (70℃ heat) (40 µg/Lane)
Lane 5: Rat embryo tissue lysate (40 µg/Lane)
Predicted band size: 41 kDa
Observed band size: 40-50kDa
Exposure time: Lane 1-4: 2 minutes; Lane 5: 27 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722180) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
