Estrogen Receptor alpha Rabbit Polyclonal Antibody (ER1803-83)

UNIPROT #

P03372

PROTEIN NAME

Estrogen Receptor alpha

SYNONYMS

DKFZp686N23123 antibody;ER alpha antibody;ER antibody;ER-alpha antibody;Era antibody;ESR antibody;ESR1 antibody;ESR1_HUMAN antibody;ESRA antibody;Estradiol receptor antibody;Estrogen nuclear receptor alpha antibody;Estrogen receptor 1 antibody;Estrogen receptor alpha 3*,4,5,6,7*/822 isoform antibody;Estrogen receptor alpha antibody;Estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform antibody;Estrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform antibody;Estrogen receptor alpha delta 4 +49 isoform antibody;Estrogen receptor alpha delta 4*,5,6,7*/654 isoform antibody;Estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform antibody;Estrogen receptor alpha E1 E2 1 2 antibody;Estrogen receptor alpha E1 N2 E2 1 2 antibody;Estrogen receptor antibody;ESTRR antibody;NR3A1 antibody;Nuclear receptor subfamily 3 group A member 1 antibody

SEQUENCE SIMILARITIES

Belongs to the nuclear hormone receptor family. NR3 subfamily.

TISSUE SPECIFICITY

Widely expressed. Isoform 3 is not expressed in the pituitary gland.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by cyclin A/CDK2 and CK1. Phosphorylation probably enhances transcriptional activity. Self-association induces phosphorylation. Dephosphorylation at Ser-118 by PPP5C inhibits its transactivation activity. Phosphorylated by LMTK3 in vitro.; Glycosylated; contains N-acetylglucosamine, probably O-linked.; Ubiquitinated; regulated by LATS1 via DCAF1 it leads to ESR1 proteasomal degradation. Deubiquitinated by OTUB1.; Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization. Demethylated by JMJD6 at Arg-260.; Palmitoylated (isoform 3). Not biotinylated (isoform 3).; Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation, but not for signaling mediated by the nuclear hormone receptor.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus. Cell membrane. Golgi apparatus.

FUNCTION

Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades.