SFRP1 Recombinant Rabbit Monoclonal Antibody [JA11-68] (ET1704-29)
Catalog# ET1704-29
Rabbit Monoclonal to SFRP1
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
-
Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of SFRP1 on different lysates with Rabbit anti-SFRP1 antibody (ET1704-29) at 1/1,000 dilution.
Lane 1: SH-SY5Y cell lysate (20 µg/Lane)
Lane 2: SK-MEL-28 cell lysate (20 µg/Lane)
Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
Lane 4: Mouse kidney tissue lysate (40 µg/Lane)
Predicted band size: 35 kDa
Observed band size: 35 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-29) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: SH-SY5Y cell lysate (20 µg/Lane)
Lane 2: SK-MEL-28 cell lysate (20 µg/Lane)
Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
Lane 4: Mouse kidney tissue lysate (40 µg/Lane)
Predicted band size: 35 kDa
Observed band size: 35 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-29) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.