Wnt3a Rabbit Polyclonal Antibody (HA500193)
Catalog# HA500193
Rabbit Polyclonal to Wnt3a
-
WB
-
IF-Cell
-
IHC-P
-
Human
Western blot analysis of Wnt3a on different lysates with Rabbit anti-Wnt3a antibody (HA500193) at 1/1,000 dilution.
Lane 1: 293T-NT cell lysate
Lane 2: 293T-OE-Wnt3a cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 39 kDa
Observed band size: 36 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500193) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
Wnt3a Rabbit Polyclonal Antibody (HA500193)
Immunogen
Synthetic peptide within human wnt3a aa 241-290.
Host
Rabbit
Positive Control
Human placenta tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
-
WB
-
1:1,000
-
IF-Cell
-
1:500
-
IHC-P
-
1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 39 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Wnt3a
SYNONYMS
Protein Wnt 3a Precursor antibody;Protein Wnt-3a antibody;Wingless type MMTV integration site family member 3A antibody;Wnt 3a antibody;wnt3a antibody;WNT3A protein antibody;WNT3A_HUMAN antibody
SUBCELLULAR LOCATION
Secreted, extracellular space, extracellular matrix.
FUNCTION
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region.
CITATIONS
-
Yanli Chang ,et al
Palmitoylation regulates myelination by modulating the ZDHHC3-Cadm4 axis in the central nervous system
-
WB
-
Mouse
-
Citation
-
-
Sun Mingjun,et al
Synergism of Fusobacterium periodonticum and N-nitrosamines promote the formation of EMT subtypes in ESCC by modulating Wnt3a palmitoylation
-
IHC
-
Mouse
-
Citation
-
