DDAH2 Recombinant Rabbit Monoclonal Antibody [JB59-33] (ET7107-41)
Catalog# ET7107-41
Rabbit Monoclonal to DDAH2
Tested for
-
WB
-
IP
Reacts with
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Human
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Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of DDAH2 on different lysates with Rabbit anti-DDAH2 antibody (ET7107-41) at 1/2,000 dilution.
Lane 1: Rat heart tissue lysate
Lane 2: Mouse heart tissue lysate
Lane 3: Mouse lung tissue lysate
Lane 4: Human lung tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 30 kDa
Observed band size: 30 kDa
Exposure time: 2 minutes;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-41) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Lane 1: Rat heart tissue lysate
Lane 2: Mouse heart tissue lysate
Lane 3: Mouse lung tissue lysate
Lane 4: Human lung tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 30 kDa
Observed band size: 30 kDa
Exposure time: 2 minutes;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-41) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.