iNOS Recombinant Rabbit Monoclonal Antibody [JE59-61] (HA721583)
Catalog# HA721583
Rabbit Monoclonal to iNOS
Tested for
-
WB
-
IF-Cell
Reacts with
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Human
-
Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
This product has been cited in peer reviewed publications, see list HERE
Western blot analysis of iNOS on different lysates with Rabbit anti-iNOS antibody (HA721583) at 1/1,000 dilution.
Lane 1: RAW264.7 whole cell lysate
Lane 2: RAW264.7 treated with 1μg/mL LPS for 24 hours whole cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 131 kDa
Observed band size: 131 kDa
Exposure time: 15 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721583) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: RAW264.7 whole cell lysate
Lane 2: RAW264.7 treated with 1μg/mL LPS for 24 hours whole cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 131 kDa
Observed band size: 131 kDa
Exposure time: 15 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721583) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
