GABA B Receptor 1 Recombinant Rabbit Monoclonal Antibody [PSH03-87] (HA722053)
Catalog# HA722053
Rabbit Monoclonal to GABA B Receptor 1
Tested for
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WB
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IF-Tissue
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IHC-P
Reacts with
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Human
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Mouse
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Rat
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Cynomolgus monkey
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Pig
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of GABA B Receptor 1 on different lysates with Rabbit anti-GABA B Receptor 1 antibody (HA722053) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.
Lane 1: Human brain tissue lysate
Lane 2: Mouse brain tissue lysate (hot lysis)
Lane 3: Rat brain tissue lysate (no heat)
Lane 4: Mouse cerebellum tissue lysate (70℃ heat)
Lane 5: Rat cerebellum tissue lysate
Lane 6: Mouse skeletal muscle tissue lysate (no heat) (negative)
Lane 7: Rat skeletal muscle tissue lysate (no heat) (negative)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 40 µg/Lane.
Predicted band size: 108 kDa
Observed band size: 95-108 kDa
Exposure time: Lane 1-7 (left): 28 seconds; Lane 1-5 (right): 2 minutes 34 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722053) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Human brain tissue lysate
Lane 2: Mouse brain tissue lysate (hot lysis)
Lane 3: Rat brain tissue lysate (no heat)
Lane 4: Mouse cerebellum tissue lysate (70℃ heat)
Lane 5: Rat cerebellum tissue lysate
Lane 6: Mouse skeletal muscle tissue lysate (no heat) (negative)
Lane 7: Rat skeletal muscle tissue lysate (no heat) (negative)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 40 µg/Lane.
Predicted band size: 108 kDa
Observed band size: 95-108 kDa
Exposure time: Lane 1-7 (left): 28 seconds; Lane 1-5 (right): 2 minutes 34 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722053) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.