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GCLM Recombinant Rabbit Monoclonal Antibody [JM39-61] (ET1705-87)

Price: Regular price $385.00

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Catalog# ET1705-87

Rabbit Monoclonal to GCLM

Tested for

  • WB

  • IF-Tissue

  • IHC-P

  • IP

  • IHC-Fr

Reacts with

  • Human

  • Mouse

  • Rat

Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
This product has been cited in peer reviewed publications, see list HERE

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Specifications

Product Type

Recombinant Rabbit monoclonal primary

Product Name

GCLM Recombinant Rabbit Monoclonal Antibody [JM39-61] (ET1705-87)

Immunogen

Recombinant protein within human gclm aa 225-274 / 274.

Host

Rabbit

Positive Control

A431 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, HeLa cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate, human liver tissue, human lung tissue, mouse liver tissue, rat liver tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM39-61

RRID

AB_3070615

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • IF-Tissue

  • 1:50-1:200

  • IHC-P

  • 1:200-1:1,000

  • IP

  • Use at an assay dependent concentration.

  • IHC-Fr

  • 1:50

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1ug/ul

Purification

Protein A affinity purified.

Molecular Weight

Predicted band size: 31 kDa

Isotype

IgG

TARGET

UNIPROT #

P48507

PROTEIN NAME

GCLM

SYNONYMS

Gamma ECS regulatory subunit antibody;Gamma-ECS regulatory subunit antibody;Gamma-glutamylcysteine synthetase regulatory subunit antibody;GCLM antibody;GCS light chain antibody;GLCLR antibody;Glutamate cysteine ligase regulatory subunit antibody;Glutamate--cysteine ligase modifier subunit antibody;Glutamate--cysteine ligase regulatory subunit antibody;GSC light chain antibody;GSH0_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the aldo/keto reductase family. Glutamate--cysteine ligase light chain subfamily.

TISSUE SPECIFICITY

In all tissues examined. Highest levels in skeletal muscle.

SUBCELLULAR LOCATION

Cytosol.

FUNCTION

Gamma-glutamylcysteine synthetase (γ-GCS) is the rate limiting enzyme for glutathione (L-gamma-glutamyl-L-cysteinylglycine, GSH) synthesis. GSH is ubiquitous in mammalian cells as a vital intra- and extracellular protective antioxidant. γ-GCS is a heterodimer of a heavy catalytic subunit and a light regulatory subunit that is responsive to inflammation, phenolic antioxidants, heat shock, oxidants and cytokines. The human gamma-GCS gene encoding the 367 amino acid catalytic subunit maps to chromosome 6p12. The human γ-GCS gene encoding the regulatory subunit maps to chromosome 1p22-p21. The two subunits of γ-GCS form a heterodimeric zinc metalloprotein that gains activity through formation of a reversible disulfide bond.

CITATIONS

  • Sun, Y., He, Y., Tong, J., Liu, D., Zhang, H., He, T., & Bi, Y.

    All-trans retinoic acid inhibits the malignant behaviors of hepatocarcinoma cells by regulating ferroptosis

  • H1N1 influenza virus infection through NRF2-KEAP1-GCLC pathway induces ferroptosis in nasal mucosal epithelial cells

  • A reactive oxygen species–related signature to predict prognosis and aid immunotherapy in clear cell renal cell carcinoma

  • Wu, J., Feng, Z., Chen, L., Li, Y., Bian, H., Geng, J., Zheng, Z. H., Fu, X., Pei, Z., Qin, Y., Yang, L., Zhao, Y., Wang, K., Chen, R., He, Q., Nan, G., Jiang, X., Chen, Z. N., & Zhu, P.

    TNF antagonist sensitizes synovial fibroblasts to ferroptotic cell death in collagen-induced arthritis mouse models

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