Niemann Pick C1 Recombinant Rabbit Monoclonal Antibody [JB87-33] (ET7107-57)
Catalog# ET7107-57
Rabbit Monoclonal to Niemann Pick C1
Tested for
-
WB
-
IF-Cell
-
IF-Tissue
-
IHC-P
-
FC
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
All lanes: Western blot analysis of Niemann Pick C1 with anti-Niemann Pick C1 antibody [JB87-33] (ET7107-57) at 1:1,000 dilution.
Lane 1: Wild-type HEK293 whole cell lysate (20 µg).
Lane 2: Niemann Pick C1 knockout HEK293 whole cell lysate (20 µg).
ET7107-57 was shown to specifically react with Niemann Pick C1 in wild-type HEK293 cells. No band was observed when Niemann Pick C1 knockout sample was tested. Wild-type and Niemann Pick C1 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET7107-57, 1/1,000) and Loading control antibody (Rabbit anti-HSP90, ET1605-56, 1/10,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Lane 1: Wild-type HEK293 whole cell lysate (20 µg).
Lane 2: Niemann Pick C1 knockout HEK293 whole cell lysate (20 µg).
ET7107-57 was shown to specifically react with Niemann Pick C1 in wild-type HEK293 cells. No band was observed when Niemann Pick C1 knockout sample was tested. Wild-type and Niemann Pick C1 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET7107-57, 1/1,000) and Loading control antibody (Rabbit anti-HSP90, ET1605-56, 1/10,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.