PAX8 Recombinant Mouse Monoclonal Antibody [6G5-R] (HA601270)
Catalog# HA601270
Mouse Monoclonal to PAX8
Tested for
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WB
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IHC-P
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IF-Cell
Reacts with
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Human
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Mouse
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Monkey
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
This product has been cited in peer reviewed publications, see list HERE
Western blot analysis of PAX8 on different lysates with Mouse anti-PAX8 antibody (HA601270) at 1/1,000 dilution.
Lane 1: NIH:OVCAR-3 cell lysate
Lane 2: Jurkat cell lysate (negative)
Lane 3: COS-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 1 minute 40 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601270) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: NIH:OVCAR-3 cell lysate
Lane 2: Jurkat cell lysate (negative)
Lane 3: COS-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 1 minute 40 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601270) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
