PRPF8 Recombinant Rabbit Monoclonal Antibody [JG57-38] (ET7108-18)

UNIPROT #

Q6P2Q9

PROTEIN NAME

PRPF8

SYNONYMS

220 kDa U5 snRNP specific protein antibody;220 kDa U5 snRNP-specific protein antibody;Apoptosis regulated protein 1 antibody;Apoptosis regulated protein 2 antibody;HPRP8 antibody;p220 antibody;Pre mRNA processing factor 8 antibody;Pre mRNA-processing factor 8, S. cerevisiae, homolog of antibody;Pre-mRNA-processing-splicing factor 8 antibody;Precursor mRNA processing protein antibody;PRP8 antibody;PRP8 homolog antibody;PRP8 pre mRNA processing factor 8 homolog antibody;PRP8_HUMAN antibody;PRPC8 antibody;Prpf8 antibody;Retinitis pigmentosa 13 (autosomal dominant) antibody;RP13 antibody;SNRNP220 antibody;Splicing factor Prp8 antibody;U5 snRNP specific protein antibody;U5 snRNP specific protein (220 kD), ortholog of S. cerevisiae Prp8p antibody

TISSUE SPECIFICITY

Widely expressed.

SUBCELLULAR LOCATION

Nucleus. Spliceosome.

FUNCTION

PRP8, also designated pre-mRNA-processing-splicing factor 8, is a highly conserved nuclear protein and a central component of the catalytic core of the spliceosome, where it may be involved in various molecular rearrangements. PRP8, which is widely expressed, plays a role in transesterification reactions that regulate splicesome-induced pre-mRNA splicing. Specifically, PRP8 interacts with the GU dinucleotide at the 5' splice site (5'SS) and forms a specific UV-inducible cross-link. It also interacts functionally with the 3'SS, affecting the efficiency of the second catalytic step. PRP8 may play a role in the first transesterification step, as PRP8 mutations that prohibit negative regulation of PRP28 or PRP44/Brr2 subsequently block U4 activation. In addition, PRP8 interacts with a conserved region of U6 that is instrumental in the formation of the catalytic core of the spliceosome.