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Gasdermin D (N terminal) Recombinant Rabbit Monoclonal Antibody [PD00-18] (HA721144)

Price: Regular price $205.00

Volume

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Catalog# HA721144-50UL

Rabbit Monoclonal to Gasdermin D (N terminal)

Tested for

  • WB

  • IHC-P

  • IF-Cell

Reacts with

  • Human

  • Mouse

  • Rat

Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
This product has been cited in peer reviewed publications, see list HERE

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Specifications

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Gasdermin D (N terminal) Recombinant Rabbit Monoclonal Antibody [PD00-18] (HA721144)

Immunogen

Recombinant protein within gasdermin d full length protein.

Host

Rabbit

Positive Control

SiHa cell lysate, Jurkat cell lysate, THP-1 cell lysate, PC-3M cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, L6 cell lysate, THP-1 treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours then add 1μg/mL BFA for 3 hours cell lysate, mouse intestine tissue, L6.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

PD00-18

RRID

AB_3072266

APPLICATION DILUTION

  • WB

  • 1:2,000-1:5,000

  • IHC-P

  • 1:1,000

  • IF-Cell

  • 1:100

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1ug/ul

Purification

Protein A affinity purified.

Molecular Weight

Predicted band size: 53/30 kDa

Isotype

IgG

TARGET

UNIPROT #

P57764

PROTEIN NAME

Gasdermin D (N terminal)

SYNONYMS

1810036L03Rik antibody;DF 5L antibody;DF5L antibody;DFNA 5L antibody;DFNA5L antibody;FKSG 10 antibody;FKSG10 antibody;FLJ12150 antibody;Gasdermin antibody;Gasdermin D antibody;Gasdermin domain containing 1 antibody;Gasdermin domain containing protein 1 antibody;Gasdermin domain-containing protein 1 antibody;Gasdermin-D antibody;GasderminD antibody;GSDMD antibody;GSDMD_HUMAN antibody;GSDMDC 1 antibody;GSDMDC1 antibody

SUBCELLULAR LOCATION

Cell membrane, Secreted.

FUNCTION

Gasdermin-D, N-terminal: Promotes pyroptosis in response to microbial infection and danger signals. Produced by the cleavage of gasdermin-D by inflammatory caspases CASP1 or CASP4 in response to canonical, as well as non-canonical (such as cytosolic LPS) inflammasome activators. After cleavage, moves to the plasma membrane where it strongly binds to inner leaflet lipids, including monophosphorylated phosphatidylinositols, such as phosphatidylinositol 4-phosphate, bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate, as well as phosphatidylinositol (3,4,5)-bisphosphate, and more weakly to phosphatidic acid and phosphatidylserine. Homooligomerizes within the membrane and forms pores of 10 - 15 nanometers (nm) of inner diameter, possibly allowing the release of mature IL1B and triggering pyroptosis . Exhibits bactericidal activity. Gasdermin-D, N-terminal released from pyroptotic cells into the extracellular milieu rapidly binds to and kills both Gram-negative and Gram-positive bacteria, without harming neighboring mammalian cells, as it does not disrupt the plasma membrane from the outside due to lipid-binding specificity.

CITATIONS

  • Dongyan Zheng,et al

    Pyroptosis mediated by Parkin-NLRP3 negative feedback loop contributed to Parkinson's disease induced by rotenone

  • Li, Q., Hong, Y., Chen, J., Zhou, X., Tian, X., Yu, Y., Shen, L., Long, C., Cai, M., Wu, S., & Wei, G.

    Hypoxia-Induced HIF-1α Expression Promotes Neurogenic Bladder Fibrosis via EMT and Pyroptosis

  • Fan Yujie,et al

    Coordination-Driven Nanomedicine Mitigates One-Lung Ventilation-Induced Lung Injury via Radicals Scavenging and Cell Pyroptosis Inhibition

  • Inhibition of the NF-κB Signaling Pathway Alleviates Pyroptosis in Bladder Epithelial Cells and Neurogenic Bladder Fibrosis

  • Libo Luo,et al

    Lipotoxicity-induced upregulation of FIS1 exacerbates mitochondrial fragmentation and promotes NLRP3-dependent pyroptosis in diabetic cardiomyopathy

  • Gasdermin D mediates doxorubicin-induced cardiomyocyte pyroptosis and cardiotoxicity via directly binding to doxorubicin and changes in mitochondrial damage

  • Dendrobium Nobile Lindl. Alkaloid Suppresses NLRP3-Mediated Pyroptosis to Alleviate LPS-Induced Neurotoxicity

  • Neuroprotective effects of Jie-du-huo-xue decoction on microglia pyroptosis after cerebral ischemia and reperfusion——From the perspective of glial-vascular unit

  • Record‐High Ultrasound‐Sensitive NO Nanogenerators for Cascade Tumor Pyroptosis and Immunotherapy

  • Rosuvastatin suppresses TNF-α-induced matrix catabolism, pyroptosis and senescence via the HMGB1/NF-κB signaling pathway in nucleus pulposus cells

  • Zhang Xianzheng,et al

    Sodium chloride promotes macrophage pyroptosis and aggravates rheumatoid arthritis by activating SGK1 through GABA receptors Slc6a12

  • Weijian Yang,et al

    Sulforaphane regulation autophagy-mediated pyroptosis in autoimmune hepatitis via AMPK/mTOR pathway

  • Lulu Tang , Dongdong Zhang , Yujing Zhang,et al

    Vitamin D3 alleviates lung fibrosis of type 2 diabetic rats via SIRT3 mediated suppression of pyroptosis

  • GLP-1RAs inhibit the activation of the NLRP3 inflammasome signaling pathway to regulate mouse renal podocyte pyroptosis

  • Essential oil of Acorus tatarinowii Schott inhibits neuroinflammation by suppressing NLRP3 inflammasome activation in 3 × Tg-AD transgenic mice

  • Ergolide covalently binds NLRP3 and inhibits NLRP3 inflammasome-mediated pyroptosis

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