Phospho-AMPK alpha 2 (S345) Recombinant Rabbit Monoclonal Antibody [JJ08-19] (ET1701-37)

UNIPROT #

P54646

PROTEIN NAME

Phospho-AMPK alpha 2 (S345)

SYNONYMS

5'-AMP-activated protein kinase catalytic subunit alpha-2 antibody;AAPK2_HUMAN antibody;ACACA kinase antibody;Acetyl-CoA carboxylase kinase antibody;AMPK alpha 2 chain antibody;AMPK subunit alpha-2 antibody;AMPK2 antibody;AMPKa2 antibody;AMPKalpha2 antibody;HMGCR kinase antibody;Hydroxymethylglutaryl-CoA reductase kinase antibody;PRKAA antibody;PRKAA2 antibody;Protein kinase AMP activated alpha 2 catalytic subunit antibody;Protein kinase AMP activated catalytic subunit alpha 2 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.

POST-TRANSLATIONAL MODIFICATION

Ubiquitinated.; Phosphorylated at Thr-172 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Also phosphorylated at Thr-172 by CAMKK2; triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio. CAMKK1 can also phosphorylate Thr-172, but at much lower level. Dephosphorylated by protein phosphatase 2A and 2C (PP2A and PP2C). Phosphorylated by ULK1; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK. Dephosphorylated by PPM1A and PPM1B at Thr-172 (mediated by STK11/LKB1).

SUBCELLULAR LOCATION

Nucleus, Cytoplasm.

FUNCTION

Five-prime-AMP-activated protein kinase, known as AMPK, is a heterotrimeric complex that comprises of a catalytic α subunit, and regulatory β and γ. AMPK protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP via a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase (AMPKK), and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. The human AMPKα1 gene maps to chromosome 5p12 and encodes a 548 amino acid protein.