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Phospho-ATM (S1981) Recombinant Rabbit Monoclonal Antibody [JM93-25] (ET1705-50)

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Catalog# ET1705-50

Rabbit Monoclonal to Phospho-ATM (S1981)

Tested for

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

  • IP

  • FC

Reacts with

  • Human

  • Mouse

Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
This product has been cited in peer reviewed publications, see list HERE

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Specifications

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Phospho-ATM (S1981) Recombinant Rabbit Monoclonal Antibody [JM93-25] (ET1705-50)

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding ser1981 of human atm aa 1,940-1,989 / 3,056.

Host

Rabbit

Modification

Phospho

Modification Site

S1981

Positive Control

HeLa cell lysate, HeLa treated with 1μM Camptothecin for 1 hour cell lysate, HeLa treated with 20μM Etoposide for 2 hours cell lysate, HepG2 cell lysate, HEK-293 cell lysate, MDA-MB-231 cell lysate, HeLa cells treated with 1μM Camptothecin for 1 hour, human breast carcinoma tissue, human colon cancer tissue, HEK-293.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM93-25

RRID

AB_3070578

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • IF-Cell

  • 1:50-1:200

  • IF-Tissue

  • 1:50-1:200

  • IHC-P

  • 1:200-1,000

  • IP

  • Use at an assay dependent concentration.

  • FC

  • 1:1,000

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1ug/ul

Purification

Protein A affinity purified.

Molecular Weight

Predicted band size: 351 kDa

Isotype

IgG

TARGET

UNIPROT #

Q13315

PROTEIN NAME

Phospho-ATM (S1981)

SYNONYMS

A-T mutated antibody;A-T mutated homolog antibody;AT mutated antibody;AT1 antibody;ATA antibody;Ataxia telangiectasia mutated antibody;Ataxia telangiectasia mutated gene antibody;Ataxia telangiectasia mutated homolog (human) antibody;Ataxia telangiectasia mutated homolog antibody;ATC antibody;ATD antibody;ATDC antibody;ATE antibody;ATM antibody;ATM serine/threonine kinase antibody;ATM_HUMAN antibody;DKFZp781A0353 antibody;MGC74674 antibody;OTTHUMP00000232981 antibody;Serine protein kinase ATM antibody;Serine-protein kinase ATM antibody;Serine/threonine-protein kinase ATM antibody;Tefu antibody;TEL1 antibody;TEL1, telomere maintenance 1, homolog antibody;TELO1 antibody;Telomere fusion protein antibody

SEQUENCE SIMILARITIES

Belongs to the PI3/PI4-kinase family. ATM subfamily.

TISSUE SPECIFICITY

Found in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, spleen, thymus, testis, ovary, small intestine, colon and leukocytes.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by NUAK1/ARK5. Autophosphorylation on Ser-367, Ser-1893, Ser-1981 correlates with DNA damage-mediated activation of the kinase. During the late stages of DNA damage response, dephosphorylated following deacetylation by SIRT7, leading to ATM deactivation.; Acetylation, on DNA damage, is required for activation of the kinase activity, dimer-monomer transition, and subsequent autophosphorylation on Ser-1981. Acetylated in vitro by KAT5/TIP60. Deacetylated by SIRT7 during the late stages of DNA damage response, promoting ATM dephosphorylation and subsequent deactivation.

SUBCELLULAR LOCATION

Nucleus. Cytoplasmic vesicle.

FUNCTION

ATM serine/threonine kinase, symbol ATM, is a serine/threonine protein kinase that is recruited and activated by DNA double-strand breaks. It phosphorylates several key proteins that initiate activation of the DNA damage checkpoint, leading to cell cycle arrest, DNA repair or apoptosis. Several of these targets, including p53, CHK2, BRCA1, NBS1 and H2AX are tumor suppressors. The ATM-mediated DNA damage response consists of a rapid and a delayed response. The protein kinase ATM may also be involved in mitochondrial homeostasis, as a regulator of mitochondrial autophagy (mitophagy) whereby old, dysfunctional mitochondria are removed. Increased ATM activity also occurs in viral infection where ATM is activated early during dengue virus infection as part of autophagy induction and ER stress response.

CITATIONS

  • Disulfiram enhances the antitumor activity of cisplatin by inhibiting the Fanconi anemia repair pathway

  • Fu, H., Liu, R., Jia, Z., Li, R., Zhu, F., Zhu, W., Shao, Y., Jin, Y., Xue, Y., Huang, J., Luo, K., Gao, X., Lu, H., & Zhou, Q.

    Poly(ADP-ribosylation) of P-TEFb by PARP1 disrupts phase separation to inhibit global transcription after DNA damage

  • Nanoengineered Gallium Ion Incorporated Formulation for Safe and Efficient Reversal of PARP Inhibition and Platinum Resistance in Ovarian Cancer

  • Xia, Z., Li, M., Hu, M., Lin, Y., Atteh, L. L., Fu, W., Gao, L., Bai, M., Huang, C., Yue, P., Liu, Y., & Meng, W.

    Phosphoproteomics reveals that cinobufotalin promotes intrahepatic cholangiocarcinoma cell apoptosis by activating the ATM/CHK2/p53 signaling pathway

  • A novel and potent dihydroorotate dehydrogenase inhibitor suppresses the proliferation of colorectal cancer by inducing mitochondrial dysfunction and DNA damage

  • Wang, M., Xu, H., Li, T., Li, K., Zhang, Q., Chen, S., Zhao, L., Chen, J., & Chen, X.

    Sonodynamic therapy of glioblastoma mediated by platelets with ultrasound-triggered drug release

  • Wang Quanxin,et al

    Taraxasterol enhanced bladder cancer cells radiosensitivity via inhibiting the COX-2/PGE2/JAK2/STAT3/MMP pathway

  • TPP1 inhibits DNA damage response and chemosensitivity in esophageal cancer

  • Yang Liuqing,et al

    Exploring the Anti-Ovarian Aging Mechanism of He's Yangchao Formula: Insights from Multi-Omics Analysis in Naturally Aged Mice

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