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Phospho-JAK2 (Y1007+Y1008) Recombinant Rabbit Monoclonal Antibody [SY24-03] (ET1607-34)

Price: Regular price $385.00

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Catalog# ET1607-34

Rabbit Monoclonal to Phospho-JAK2 (Y1007+Y1008)

Tested for

  • WB

  • IF-Cell

  • IHC-P

  • IP

Reacts with

  • Human

  • Mouse

  • Rat

Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
This product has been cited in peer reviewed publications, see list HERE

Safety datasheet

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Specifications

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Phospho-JAK2 (Y1007+Y1008) Recombinant Rabbit Monoclonal Antibody [SY24-03] (ET1607-34)

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding tyr1007 and 1008 of human jak2.

Host

Rabbit

Modification

Phospho

Modification Site

Y1007+Y1008

Positive Control

HeLa treated with 1mM sodium orthovanadate for 30 minutes cell lysate, human lung carcinoma tissue, human tonsil tissue, human kidney tissue, rat kidney tissue, mouse kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SY24-03

RRID

AB_3069759

APPLICATION DILUTION

  • WB

  • 1:1,000

  • IF-Cell

  • 1:50-1:200

  • IHC-P

  • 1:200-1:500

  • IP

  • Use at an assay dependent concentration.

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1ug/ul

Purification

Protein A affinity purified.

Molecular Weight

Predicted band size: 131 kDa

Isotype

IgG

TARGET

UNIPROT #

O60674

PROTEIN NAME

Phospho-JAK2 (Y1007+Y1008)

SYNONYMS

JAK 2 antibody;JAK-2 antibody;JAK2 antibody;JAK2_HUMAN antibody;Janus Activating Kinase 2 antibody;Janus kinase 2 (a protein tyrosine kinase) antibody;Janus kinase 2 antibody;JTK 10 antibody;JTK10 antibody;kinase Jak2 antibody;OTTHUMP00000043260 antibody;THCYT3 antibody;Tyrosine protein kinase JAK2 antibody;Tyrosine-protein kinase JAK2 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.

TISSUE SPECIFICITY

Ubiquitously expressed throughout most tissues.

POST-TRANSLATIONAL MODIFICATION

Autophosphorylated, leading to regulate its activity. Leptin promotes phosphorylation on tyrosine residues, including phosphorylation on Tyr-813 (By similarity). Autophosphorylation on Tyr-119 in response to EPO down-regulates its kinase activity (By similarity). Autophosphorylation on Tyr-868, Tyr-966 and Tyr-972 in response to growth hormone (GH) are required for maximal kinase activity (By similarity). Also phosphorylated by TEC (By similarity). Phosphorylated on tyrosine residues in response to interferon gamma signaling. Phosphorylated on tyrosine residues in response to a signaling cascade that is activated by increased cellular retinol.

SUBCELLULAR LOCATION

Nucleus, Endomembrane system, Cytoplasm.

FUNCTION

JAK2 (Janus Kinase 2) belongs to the emerging family of non-receptor Janus tyrosine kinases, which regulate a spectrum of cellular functions downstream of activated cytokine receptors in the lympho-hematopoietic system. Immuno-logical stimuli, such as interferons and cytokines, induce recruitment of Stat transcription factors to cytokine receptor-associated JAK2. JAK2 then phosphorylates proximal Stat factors, which subsequently dimerize, translocate to the nucleus and bind to cis elements upstream of target gene promoters to regulate transcription. The canonical JAK-Stat pathway is integral to maintaining a normal immune system by stimulating proliferation, differentiation, survival, and host resistance to pathogens. Altering JAK-Stat signaling to reduce cytokine induced pro-inflammatory responses represents an attractive target for anti-inflammatory therapies. Within the JAK2 kinase domain, there is a region that has considerable sequence homology to the regulatory region of the insulin receptor. Among a variety of sites, Tyrosines 1007 and 1008 are sites of trans- or autophosphorylation in vivo and in in vitro kinase reactions.

CITATIONS

  • Wang Yingying,et al

    Linderae Radix extract attenuates ulcerative colitis by inhibiting the JAK/STAT signaling pathway

  • Gu, X., Miao, Z., Wang, Y., Yang, Y., Yang, T., & Xu, Y.

    New Baitouweng decoction combined with fecal microbiota transplantation alleviates DSS-induced colitis in rats by regulating gut microbiota metabolic homeostasis and the STAT3/NF-κB signaling pathway

  • Ren, Q., Wang, B., Guo, F., Huang, R., Tan, Z., Ma, L., & Fu, P.

    Natural Flavonoid Pectolinarigenin Alleviated Hyperuricemic Nephropathy via Suppressing TGFβ/SMAD3 and JAK2/STAT3 Signaling Pathways

  • Zhang Jie,et al

    Cediranib enhances the transcription of MHC-I by upregulating IRF-1

  • Novel synthetic 4-chlorobenzoyl berbamine inhibits c-Myc expression and induces apoptosis of diffuse large B cell lymphoma cells.

  • Liu, Y., Hao, Y., Zhao, H., Zhang, Y., Cheng, D., Zhao, L., Peng, Y., Lu, Y., & Li, Y.

    PlexinA1 activation induced by β2-AR promotes epithelial-mesenchymal transition through JAK-STAT3 signaling in human gastric cancer cells

  • Deng, L., Zhang, X., Xiang, X., Xiong, R., Xiao, D., Chen, Z., Liu, K., & Feng, G.

    NANOG Promotes Cell Proliferation, Invasion, and Stemness via IL-6/STAT3 Signaling in Esophageal Squamous Carcinoma

  • Li Weijing,et al

    Dexmedetomidine hydrochloride plus sufentanil citrate inhibits glucose metabolism and epithelial‑mesenchymal transition in human esophageal squamous carcinoma KYSE30 cells by modulating the JAK/STAT3/HIF‑1α axis

  • Fu, Y., Peng, Y., Zhao, S., Mou, J., Zeng, L., Jiang, X., Yang, C., Huang, C., Li, Y., Lu, Y., Wu, M., Yang, Y., Kong, T., Lai, Q., Wu, Y., Yao, Y., Wang, Y., Gou, L., & Yang, J.

    Combination Foretinib and Anti-PD-1 Antibody Immunotherapy for Colorectal Carcinoma. Frontiers in cell and developmental biology, 9, 689727.

  • IL-4/IL-13 upregulates Sonic hedgehog expression to induce allergic airway epithelial remodeling

  • Acute myeloid leukemia (AML)‐derived mesenchymal stem cells induce chemoresistance and epithelial–mesenchymal transition‐like program in AML through IL‐6/JAK2/STAT3 signaling

  • Li, Y., Guo, F., Huang, R., Ma, L., & Fu, P.

    Natural flavonoid pectolinarigenin alleviated kidney fibrosis via inhibiting the activation of TGFβ/SMAD3 and JAK2/STAT3 signaling. International immunopharmacology, 91, 107279.

  • Ren, Q., Tao, S., Guo, F., Wang, B., Yang, L., Ma, L., & Fu, P.

    Natural flavonol fisetin attenuated hyperuricemic nephropathy via inhibiting IL-6/JAK2/STAT3 and TGF-β/SMAD3 signaling. Phytomedicine : international journal of phytotherapy and phytopharmacology, 87, 153552.

  • Yang Boshen,et al

    Ruxolitinib-based senomorphic therapy mitigates cardiomyocyte senescence in septic cardiomyopathy by inhibiting the JAK2/STAT3 signaling pathway

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