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Biotin Conjugated Goat anti-rabbit IgG Goat Polyclonal Antibody (HA1101)

Price: Regular price $349.00

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Catalog# HA1101

Goat Polyclonal to Goat anti-rabbit IgG

Tested for

  • WB

  • ELISA

  • IHC-P

  • IF-Tissue

  • FC

  • IF-Cell

Reacts with

  • Rabbit

Conjugated to Biotin
This product has been cited in peer reviewed publications, see list HERE

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Specifications

Product Type

Goat polyclonal secondary

Product Name

Biotin Conjugated Goat anti-rabbit IgG Goat Polyclonal Antibody (HA1101)

Immunogen

Rabbit igg (h+l).

Specificity

To minimize cross-reactivity, the biotin conjugated goat anti-rabbit igg (h+l) antibodies have been highly cross-adsorbed against human igg and mouse igg.

Host

Goat

Conjugation

Biotin

Clonality

Polyclonal

APPLICATION DILUTION

  • WB

  • 1:5,000-1:20,000

  • ELISA

  • 1:20,000-1:400,000

  • IHC-P

  • 1:500-1:5,000

  • IF-Tissue

  • 1:200-1:1,000

  • FC

  • 1:200-1:1,000

  • IF-Cell

  • 1:200

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 1%BSA, 50%Glycerol.

Concentration

1ug/ul

Purification

Immunogen affinity purified.

Isotype

IgG

TARGET

PROTEIN NAME

Goat anti-rabbit IgG

FUNCTION

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.Biotin-SP is our trade name for biotin with a 6-atom spacer positioned between biotin and the protein to which it is conjugated. When Biotin-SP-conjugated antibodies are used in enzyme immunoassays, there is an increase in sensitivity compared to biotin-conjugated antibodies without the spacer. This is especially notable when Biotin-SP conjugated antibodies are used with alkaline phosphatase-conjugated streptavidin. Apparently, the long spacer extends the biotin moiety away from the antibody surface, making it more accessible to binding sites on streptavidin. Biotinylated antibodies require an additional reagent for visualization. We offer streptavidin and Mouse Anti-Biotin conjugated to fluorophores and enzymes.

CITATIONS

  • Borneol-modified tanshinone IIA liposome improves cerebral ischemia reperfusion injury by suppressing NF-κB and ICAM-1 expression

  • Wang Dongmei,et al

    FOXA3 regulates cholesterol metabolism to compensate for low uptake during the progression of lung adenocarcinoma

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