CD80/B7-1 Rabbit Polyclonal Antibody (1007-8)
Catalog# 1007-8
Rabbit Polyclonal to CD80/B7-1
Tested for
-
WB
-
IF-Cell
Reacts with
-
Human
Western blot analysis of CD80 on Raji cell lysates with Rabbit anti-CD80 antibody (1007-8) at 1/1,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 33 kDa
Observed band size: 55-75 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (1007-8) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 33 kDa
Observed band size: 55-75 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (1007-8) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.