iFluor™ 488 Conjugated Goat anti-mouse IgG Goat Polyclonal Antibody (HA1125)
Catalog# HA1125
Goat Polyclonal to Goat anti-mouse IgG
Tested for
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IF-Cell
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IF-Tissue
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FC
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IHC-Fr
Reacts with
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Mouse
Conjugated to iFluor™ 488
This product has been cited in peer reviewed publications, see list HERE
Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling beta Ⅲ tubulin (M0805-8) and Synaptophysin (ET1606-56).
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies beta Ⅲ tubulin (M0805-8, green) at 1/200 dilution and Synaptophysin (ET1606-56, red) at 1/200 dilution at +4℃ overnight, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) and Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibodies at 1/1,000 dilution. DAPI was used as nuclear counterstain.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies beta Ⅲ tubulin (M0805-8, green) at 1/200 dilution and Synaptophysin (ET1606-56, red) at 1/200 dilution at +4℃ overnight, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) and Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibodies at 1/1,000 dilution. DAPI was used as nuclear counterstain.
