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iFluor™ 594 Conjugated Goat anti-Rat IgG(H+L) Goat Polyclonal Antibody (HA1136)

Price: Regular price $349.00

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Catalog# HA1136

Goat Polyclonal to Goat anti-Rat IgG(H+L)

Tested for

  • IF-Tissue

  • IHC-Fr

  • IF-Cell

  • FC

Reacts with

  • Rat

Conjugated to iFluor™ 594

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Specifications

Product Type

Goat polyclonal secondary

Product Name

iFluor™ 594 Conjugated Goat anti-Rat IgG(H+L) Goat Polyclonal Antibody (HA1136)

Immunogen

Rat igg(h+l).

Host

Goat

Conjugation

Ifluor™ 594

Clonality

Polyclonal

APPLICATION DILUTION

  • IF-Tissue

  • 1:500

  • IHC-Fr

  • 1:500

  • IF-Cell

  • 1:500

  • FC

  • 1:500

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS

Concentration

2ug/ul

Purification

Immunogen affinity purified.

Isotype

IgG

TARGET

PROTEIN NAME

Goat anti-Rat IgG(H+L)

FUNCTION

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. iFluor™ 594 dyes have fluorescence excitation and emission maxima of ~590 nm and ~610 nm respectively. iFluor™ 594 family is pH-independent from pH 3 to 11. These spectral characteristics make this new dye family an excellent alternative. iFluor™ 594 is much easier to be conjugated with RPE with much higher conjugation yield, and the resulted RPE-iFluor™ 594 tandem has better FRET efficiency. iFluor™ 594 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups.

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