iFluor™ 647 Conjugated Goat anti-mouse IgG Goat Polyclonal Antibody (HA1127)
Catalog# HA1127
Goat Polyclonal to Goat anti-mouse IgG
Tested for
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IF-Cell
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IF-Tissue
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IHC-Fr
Reacts with
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Mouse
Conjugated to iFluor™ 647
This product has been cited in peer reviewed publications, see list HERE
Immunocytochemistry analysis of Hela cells labeling Beta Ⅲ tubulin with Mouse anti-Beta Ⅲ tubulin antibody (M0805-8) at 1/400 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 3% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Beta Ⅲ tubulin antibody (M0805-8) at 1/400 dilution in 3% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Cells were fixed in 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 3% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Beta Ⅲ tubulin antibody (M0805-8) at 1/400 dilution in 3% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
