iFluor™ 647 Conjugated Goat anti-rabbit IgG Goat Polyclonal Antibody (HA1123)
Catalog# HA1123
Goat Polyclonal to Goat anti-rabbit IgG
Tested for
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IF-Cell
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IF-Tissue
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IHC-Fr
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FC
Reacts with
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Rabbit
Conjugated to iFluor™ 647
This product has been cited in peer reviewed publications, see list HERE
Immunocytochemistry analysis of Hela cells labeling Vimentin with Rabbit anti-Vimentin antibody (ET1610-39) at 1/400 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 3% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Vimentin antibody (ET1610-39) at 1/400 dilution in 3% BSA overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 647, HA1123) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Cells were fixed in 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 3% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Vimentin antibody (ET1610-39) at 1/400 dilution in 3% BSA overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 647, HA1123) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
