IQGAP1 Recombinant Rabbit Monoclonal Antibody [JG38-74] (ET7108-79)

UNIPROT #

P46940

PROTEIN NAME

IQGAP1

SYNONYMS

HUMORFA01 antibody;IQ motif containing GTPase activating protein 1 antibody;IQGA1_HUMAN antibody;Iqgap1 antibody;KIAA0051 antibody;p195 antibody;Ras GTPase activating like protein 1 antibody;Ras GTPase-activating-like protein IQGAP1 antibody;RasGAP-like with IQ motifs antibody;SAR1 antibody

TISSUE SPECIFICITY

Expressed in the placenta, lung, and kidney. A lower level expression is seen in the heart, liver, skeletal muscle and pancreas.

DEVELOPMENTAL STAGE

Expressed widely in developing cortex.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation of Ser-1443 by PKC/PRKCE prevents interaction between C1 and C2, allowing binding of nucleotide-free CDC42. Ser-1443 phosphorylation enhances the ability to promote neurite outgrowth.

SUBCELLULAR LOCATION

Cell membrane. Cytoplasm. Nucleus.

FUNCTION

IQGAP1, for IQ motif containing GTPase activating protein, is a Ras GAP-related, Actin-binding protein that interacts with the small GTPases Cdc42 and Rac1. The C-terminus of IQGAP1 is essential for interacting with Cdc42 and, in addition, IQGAP1 contains a WW domain and a predicted N-terminal coiled-coil region, which may be involved in IQGAP dimerization. Expression of IQGAP1 is highest in placenta, lung and kidney, where it co-localizes with Cdc42 to the cytoskeleton and assists with Cdc42 in mediating the regulation of cell proliferation, polarity and cell morphology. IQGAP1 regulates cadherin-mediated cell adhesion via binding to E-cadherin, b-catenin and a-catenin. This association induces the accumulation of these proteins at the site of cell-cell contact. IQGAP1 is negatively regulated by calmodulin, which binds to IQGAP1 in a calcium-dependent manner and disrupts IQGAP1 from associating with Cdc42.