Phospho-Ezrin (T567)/Radixin (T564)/Moesin (T558) Recombinant Rabbit Monoclonal Antibody [PSH05-73] (HA722440)
Catalog# HA722440
Rabbit Monoclonal to Phospho-Ezrin (T567)/Radixin (T564)/Moesin (T558)
Tested for
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WB
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IF-Cell
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IHC-P
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Phospho-Ezrin (T567)/Radixin (T564)/Moesin (T558) on different lysates with Rabbit anti-Phospho-Ezrin (T567)/Radixin (T564)/Moesin (T558) antibody (HA722440) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.
Lane 1: HeLa starved for 3 hours cell lysate
Lane 2: HeLa starved for 3 hours then add 100nM Calyculin A for 30 minutes cell lysate
Lane 3: NIH/3T3 starved for 24 hours cell lysate
Lane 4: NIH/3T3 starved for 24 hours then add 100nM Calyculin A for 30 minutes cell lysate
Lane 5: C6 cell lysate
Lane 6: C6 treated with 100ng/mL Calyculin A for 1 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 69 kDa
Observed band size: 75/80 kDa
Exposure time: 9 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722440) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa starved for 3 hours cell lysate
Lane 2: HeLa starved for 3 hours then add 100nM Calyculin A for 30 minutes cell lysate
Lane 3: NIH/3T3 starved for 24 hours cell lysate
Lane 4: NIH/3T3 starved for 24 hours then add 100nM Calyculin A for 30 minutes cell lysate
Lane 5: C6 cell lysate
Lane 6: C6 treated with 100ng/mL Calyculin A for 1 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 69 kDa
Observed band size: 75/80 kDa
Exposure time: 9 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722440) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.