IL-10RB Rabbit Polyclonal Antibody (HA500322)
Catalog# HA500322
Rabbit Polyclonal to IL-10RB
Tested for
-
WB
-
IF-Cell
-
FC
Reacts with
-
Human
Western blot analysis of IL-10RB on different lysates with Rabbit anti-IL-10RB antibody (HA500322) at 1/500 dilution.
Lane 1: 293T cell lysate
Lane 2: Jurkat cell lysate
Lane 3: Hela cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 37 kDa
Observed band size: 42 kDa (Glycoprotein)
Exposure time: 2 minutes;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500322) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Lane 1: 293T cell lysate
Lane 2: Jurkat cell lysate
Lane 3: Hela cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 37 kDa
Observed band size: 42 kDa (Glycoprotein)
Exposure time: 2 minutes;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500322) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.