SAM68 Recombinant Rabbit Monoclonal Antibody [JE47-15] (ET7109-52)

UNIPROT #

Q07666

PROTEIN NAME

SAM68

SYNONYMS

FLJ34027 antibody;GAP associated tyrosine phosphoprotein p62 antibody;GAP-associated tyrosine phosphoprotein p62 antibody;KH domain containing RNA binding signal transduction associated 1 antibody;KH domain-containing antibody;KHDR1_HUMAN antibody;Khdrbs1 antibody;p21 Ras GTPase activating protein associated p62 antibody;p21 Ras GTPase-activating protein-associated p62 antibody;p62 antibody;p68 antibody;RNA-binding antibody;Sam68 antibody;signal transduction-associated protein 1 antibody;Src associated in mitosis 68 kDa protein antibody;Src-associated in mitosis 68 kDa protein antibody

SEQUENCE SIMILARITIES

Belongs to the KHDRBS family.

TISSUE SPECIFICITY

Ubiquitously expressed in all tissue examined. Isoform 1 is expressed at lower levels in brain, skeletal muscle, and liver whereas isoform 3 is intensified in skeletal muscle and in liver.

DEVELOPMENTAL STAGE

Isoform 3 is only expressed in growth-arrested cells.

POST-TRANSLATIONAL MODIFICATION

Tyrosine phosphorylated by several non-receptor tyrosine kinases including LCK, FYN and JAK3. Also tyrosine phosphorylated by the non-receptor tyrosine kinase SRMS in an EGF-dependent manner. Negatively correlates with ability to bind RNA but required for many interactions with proteins. Phosphorylation by PTK6 negatively regulates its RNA binding ability. Phosphorylation by PTK6 at Tyr-440 dictates the nuclear localization of KHDRBS1. Phosphorylation at Tyr-387 disrupts interaction with APC. Phosphorylation at tyrosine residues by FYN inverts activity on modulation of BCL2L1 alternative splicing.; Acetylated. Positively correlates with ability to bind RNA.; Arginine methylation is required for nuclear localization. Also can affect interaction with other proteins. Inhibits interaction with Src-like SH3 domains, but not interaction with WW domains of WBP4/FBP21 AND FNBP4/FBP30.

SUBCELLULAR LOCATION

Cytoplasm. Membrane. Nucleus.

FUNCTION

KH domain-containing, RNA-binding, signal transduction-associated protein 1 is a protein that in humans is encoded by the KHDRBS1 gene. This gene encodes a member of the K homology domain-containing, RNA-binding, signal transduction-associated protein family. The encoded protein appears to have many functions and may be involved in a variety of cellular processes, including alternative splicing, cell cycle regulation, RNA 3'-end formation, tumorigenesis, and regulation of human immunodeficiency virus gene expression. Sam68 (the Src-Associated substrate in Mitosis of 68 kDa) is officially called KHDRBS1 (KH domain containing, RNA binding, signal transduction associated 1). Sam68 is a KH-type RNA binding protein that recognizes U(U/A)AA direct repeats with relative high affinity. Sam68 is predominantly nuclear and its major function in the nucleus is to regulate alternative splicing by recognizing RNA sequences neighboring the included/excluded exon(s).