14-3-3 sigma Recombinant Rabbit Monoclonal Antibody [SD2070] (ET1612-41)
Catalog# ET1612-41
Rabbit Monoclonal to 14-3-3 sigma
-
WB
-
IHC-P
-
IF-Tissue
-
Human
-
Mouse
-
Rat
Lysates/proteins at 20 µg/Lane.
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 1 minute 11 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-41) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
14-3-3 sigma Recombinant Rabbit Monoclonal Antibody [SD2070] (ET1612-41)
Immunogen
Synthetic peptide within human 14-3-3 sigmaaa 50-99 / 248.
Host
Rabbit
Positive Control
A431 cell lysates, human skin tissue, mouse skin tissue, rat skin tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SD2070
RRID
APPLICATION DILUTION
-
WB
-
1:1,000
-
IHC-P
-
1:2,000-1:10,000
-
IF-Tissue
-
1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 28 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
14-3-3 sigma
SYNONYMS
14 3 3 protein sigma antibody;14-3-3 protein sigma antibody;1433S_HUMAN antibody;Epithelial cell marker protein 1 antibody;Er antibody;HME 1 antibody;HME1 antibody;MGC143283 antibody;Mkrn3 antibody;Mme1 antibody;OTTHUMP00000004242 antibody;RP23 137L22.11 antibody;SFN antibody;SFN protein antibody;Stratifin antibody;YWHAS antibody
SEQUENCE SIMILARITIES
Belongs to the 14-3-3 family.
TISSUE SPECIFICITY
Present mainly in tissues enriched in stratified squamous keratinizing epithelium.
POST-TRANSLATIONAL MODIFICATION
Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation.
SUBCELLULAR LOCATION
Cytoplasm, Nucleus, Secreted.
FUNCTION
14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms, denoted 14-3-3 b, g, e, z, h, q and s, comprise this family of signaling intermediates. 14-3-3 s, also known as SFN, stratifin, HME1 or YWHAS, is a secreted adaptor protein that is involved in regulating both general and specific signaling pathways. Expressed predominately in stratified squamous keratinising epithelium, 14-3-3 s is able to bind and modify the activity of a large number of proteins, such as KRT17 (Keratin 17), through recognition of a phosphothreonine or phosphoserine motif. When bound to Keratin 17, for example, 14-3-3 s acts to stimulate the Akt/mTOR signaling pathway by upregulating protein synthesis and cell growth. 14-3-3 s also functions to positively mediate IGF-I-induced cell cycle progression and can bind to a variety of translation initiation factors, thus controlling mitotic translation. In response to tumor growth, 14-3-3 s positively regulates the tumor suppressor p53 and increases the rate of p53-regulated inhibition of G2/M cell cycle progression. Multiple isoforms of 14-3-3 s exist due to alternative splicing events.
