Integrin alpha 5 Recombinant Rabbit Monoclonal Antibody [JJ08-94] (ET1701-58)
Catalog# ET1701-58
Rabbit Monoclonal to Integrin alpha 5
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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IP
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FC
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Human
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Mouse
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Monkey
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Rat
Lane 1: HeLa cell lysate
Lane 2: A549 cell lysate
Lane 3: U-937 cell lysate
Lane 4: NIH/3T3 cell lysate
Lane 5: COS-1 cell lysate
Lane 6: Mouse liver tissue lysate
Lane 7: Rat liver tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 115 kDa
Observed band size: 140/19 kDa
Exposure time: 1 minute 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-58) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Integrin alpha 5 Recombinant Rabbit Monoclonal Antibody [JJ08-94] (ET1701-58)
Immunogen
Synthetic peptide within c-terminal human integrin alpha 5.
Host
Rabbit
Positive Control
HeLa cell lysate, A549 cell lysate, U-937 cell lysate, NIH/3T3 cell lysate, COS-1 cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate, NIH/3T3, human kidney tissue, human placenta tissue, human tonsil tissue, mouse endometrium tissue, Jurkat.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JJ08-94
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:2,000
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IF-Cell
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1:50-1:200
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IF-Tissue
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1:50-1:200
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IHC-P
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1:200-1:1,000
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FC
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1:50-1:100
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IP
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Use at an assay dependent concentration.
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 115 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Integrin alpha 5
SYNONYMS
CD49 antigen-like family member E antibody;CD49e antibody;Fibronectin receptor subunit alpha antibody;Fibronectin receptor, alpha subunit antibody;FNRA antibody;Integrin alpha 5 (fibronectin receptor alpha) antibody;Integrin alpha-5 antibody;Integrin alpha-5 light chain antibody;Integrin alpha-F antibody;Integrin, alpha 5 (fibronectin receptor, alpha polypeptide) antibody;ITA5_HUMAN antibody;Itga5 antibody;Very late activation protein 5, alpha subunit antibody;VLA-5 antibody;VLA5 antibody;VLA5A antibody
SEQUENCE SIMILARITIES
Belongs to the integrin alpha chain family.
POST-TRANSLATIONAL MODIFICATION
Proteolytic cleavage by PCSK5 mediates activation of the precursor.
SUBCELLULAR LOCATION
Membrane, Cell junction, Cell surface.
FUNCTION
Integrins are heterodimers composed of noncovalently associated transmembrane α and β subunits. The 16 α and 8 β subunits heterodimerize to produce more than 20 different receptors. Most integrin receptors bind ligands that are components of the extracellular matrix, including Fibronectin, Collagen and Vitronectin. Certain integrins can also bind to soluble ligands such as Fibrinogen, or to counterreceptors on adjacent cells such as the intracellular adhesion molecules (ICAMs), leading to aggregation of cells. Ligands serve to cross-link or cluster integrins by binding to adjacent integrin receptors; both receptor clustering and ligand occupancy are necessary for the activation of integrin-mediated responses. In addition to mediating cell adhesion and cytoskeletal organization, integrins function as signaling receptors. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis.
CITATIONS
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Professor Weiguo Lu
FAM83A exerts tumor-suppressive roles in cervical cancer by regulating integrins
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WB
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IHC
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IF
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Human
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Citation
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Lin, Z., Xu, Q., Miao, D., & Yu, F.
An Inflammatory Response-Related Gene Signature Can Impact the Immune Status and Predict the Prognosis of Hepatocellular Carcinoma. Frontiers in oncology, 11, 644416.
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IHC
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Human
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Citation
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