Nestin Recombinant Rabbit Monoclonal Antibody [SN06-27] (ET1611-21)

UNIPROT #

P48681

PROTEIN NAME

Nestin

SYNONYMS

ESTM 46 antibody;FLJ 21841 antibody;FLJ21841 antibody;Intermediate filament protein antibody;Nbla00170 antibody;nes antibody;NEST_HUMAN antibody;Nestin antibody

SEQUENCE SIMILARITIES

Belongs to the intermediate filament family.

TISSUE SPECIFICITY

CNS stem cells.

DEVELOPMENTAL STAGE

Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.

POST-TRANSLATIONAL MODIFICATION

Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized (By similarity).

SUBCELLULAR LOCATION

Cytoplasm, Intermediate filament.

FUNCTION

Nestin is a major intermediate filament (IF) protein of embryonic central nervous system progenitor cells. It is also a component of the dynamic IF network during muscle development, where it polymerizes with Desmin and Vimentin. Nestin co-assembles with Vimentin or a-internexin and forms heterodimer coiled-coil molecules which then further assemble into 10 nml IFs. Deletion of the IF consensus rod domain in nestin alters nestin localization in CNS precursor cells and radial glial cells in vivo. Nestin is a marker for neuroepithelial stem cells, glioma cells and tumor endothelial cells during rapid growth. During axon elongation of differen-tiation neurons, nestin localizes to the growth cones and may play a role in growth cone guidance. In the rat adrenal gland, nestin is expressed by the zona fasciculata and the zona reticularis. Nestin is also expressed by dermatomal cells and by myoblasts during the earliest stages of myogenesis.