Desmocollin 3 Recombinant Rabbit Monoclonal Antibody [JE55-86] (ET7111-26)
Catalog# ET7111-26
Rabbit Monoclonal to Desmocollin 3
Tested for
-
WB
-
IHC-P
Reacts with
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Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Desmocollin 3 on different lysates with Rabbit anti-Desmocollin 3 antibody (ET7111-26) at 1/2,000 dilution.
Lane 1: A431 cell lysate (RIPA lysis)
Lane 2: HCT 116 cell lysate (low expression) (RIPA lysis)
Lane 3: Mouse skin tissue lysate (RIPA lysis)
Lane 4: Mouse skin tissue lysate (hot lysis)
Lane 5: Rat skin tissue lysate (hot lysis)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 100 kDa
Observed band size: 100 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-26) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: A431 cell lysate (RIPA lysis)
Lane 2: HCT 116 cell lysate (low expression) (RIPA lysis)
Lane 3: Mouse skin tissue lysate (RIPA lysis)
Lane 4: Mouse skin tissue lysate (hot lysis)
Lane 5: Rat skin tissue lysate (hot lysis)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 100 kDa
Observed band size: 100 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-26) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.