Cathepsin B Recombinant Rabbit Monoclonal Antibody [JA11-02] (ET1704-03)
Catalog# ET1704-03
Rabbit Monoclonal to Cathepsin B
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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Human
Western blot analysis of Cathepsin B on different lysates with Rabbit anti-Cathepsin B antibody (ET1704-03) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: SW480 cell lysate
Lane 3: Daudi cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 38 kDa
Observed band size: 38 kDa
Exposure time: 1 minute 59 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-03) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Cathepsin B Recombinant Rabbit Monoclonal Antibody [JA11-02] (ET1704-03)
Immunogen
Recombinant protein within human cathepsin b aa 7-176 / 339.
Host
Rabbit
Positive Control
SW480 cell lysates, human liver carcinoma tissue, human liver tissue, human pancreas tissue, mouse liver tissue, mouse pancreas tissue, mouse brain tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JA11-02
RRID
APPLICATION DILUTION
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WB
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1:500-1:1,000
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IF-Cell
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1:50-1:200
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IF-Tissue
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1:50-1:200
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IHC-P
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1:50-1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 38 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Cathepsin B
SYNONYMS
Amyloid precursor protein secretase antibody;APP secretase antibody;APPS antibody;CATB_HUMAN antibody;Cathepsin B heavy chain antibody;Cathepsin B1 antibody;CathepsinB antibody;CPSB antibody;CTSB antibody;cysteine protease antibody;OTTHUMP00000116009 antibody;OTTHUMP00000229510 antibody;OTTHUMP00000229511 antibody;OTTHUMP00000229512 antibody;OTTHUMP00000229514 antibody;OTTHUMP00000229515 antibody;OTTHUMP00000229516 antibody;Preprocathepsin B antibody
SEQUENCE SIMILARITIES
Belongs to the peptidase C1 family.
TISSUE SPECIFICITY
Expressed in the stratum spinosum of the epidermis. Weak expression is detected in the stratum granulosum.
SUBCELLULAR LOCATION
Lysosome. Melanosome.
FUNCTION
The cathepsin family of proteolytic enzymes contains several diverse classes of proteases. The cysteine protease class comprises cathepsins B, L, H, K, S, and O. The aspartyl protease class is composed of cathepsins D and E. Cathepsin G is in the serine protease class. Most cathepsins are lysosomal and each is involved in cellular metabolism, participating in various events such as peptide biosynthesis and protein degradation. Cathepsin B is expressed in luminal epithelial cells, indicating that cathepsin B is a marker for secretory cell death.
CITATIONS
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Inhibition of STX17–SNAP29–VAMP8 complex formation by costunolide sensitizes ovarian cancer cells to cisplatin via the AMPK/mTOR signaling pathway
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WB
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Human
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Citation
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