Insulin Mouse Monoclonal Antibody [A6-6] (EM80714)
Catalog# EM80714
Mouse Monoclonal to Insulin
Tested for
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IHC-P
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ELISA
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IF-Tissue
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IHC-Fr
Reacts with
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Human
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Mouse
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Rat
This product has been cited in peer reviewed publications, see list HERE
Immunofluorescence analysis of frozen mouse pancreas tissue with Mouse anti-Insulin antibody (EM80714) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (EM80714, red) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Glucagon (ET1702-20, green) was stained at 1/500 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (EM80714, red) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Glucagon (ET1702-20, green) was stained at 1/500 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution.
