Insulin degrading enzyme Recombinant Rabbit Monoclonal Antibody [JJ0949] (ET1701-97)
Catalog# ET1701-97
Rabbit Monoclonal to Insulin degrading enzyme
Tested for
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WB
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IHC-P
Reacts with
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Human
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Mouse
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Rat
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Zebrafish
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Insulin degrading enzyme on different lysates with Rabbit anti-Insulin degrading enzyme antibody (ET1701-97) at 1/2,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-Insulin degrading enzyme KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 118 kDa
Observed band size: 100 kDa
Exposure time: 18 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-97) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-Insulin degrading enzyme KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 118 kDa
Observed band size: 100 kDa
Exposure time: 18 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-97) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.