IDO Rabbit Polyclonal Antibody (ER1706-94)
Catalog# ER1706-94
Rabbit Polyclonal to IDO
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Mouse
Lane 1: A549 cell lysate
Lane 2: A549 treated with 50ng/mL IFN-gamma for 24 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1706-94) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
IDO Rabbit Polyclonal Antibody (ER1706-94)
Immunogen
Recombinant protein within human ido aa 176-342.
Host
Rabbit
Positive Control
A549 treated with 50ng/mL IFN-gamma for 24 hours cell lysate, A549 cells treated with 50ng/mL IFN-gamma for 24 hours, human tonsil tissue, human spleen tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:5,000
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IF-Cell
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1:2,000
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 45 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
IDO
SYNONYMS
3-dioxygenase antibody;I23O1_HUMAN antibody;IDO 1 antibody;IDO antibody;IDO-1 antibody;IDO1 antibody;INDO antibody;indolamine 2,3 dioxygenase antibody;Indole 2 3 dioxygenase antibody;indoleamine 2 3 dioxygenase 1 antibody;indoleamine 2 3 dioxygenase antibody;Indoleamine 2,3-dioxygenase 1 antibody;Indoleamine pyrrole 2 3 dioxygenase antibody;Indoleamine-pyrrole 2 antibody
SEQUENCE SIMILARITIES
Belongs to the indoleamine 2,3-dioxygenase family.
TISSUE SPECIFICITY
Expressed in mature dendritic cells located in lymphoid organs (including lymph nodes, spleen, tonsils, Peyers's patches, the gut lamina propria, and the thymic medulla), in some epithelial cells of the female genital tract, as well as in endothelial cells of term placenta and in lung parenchyma. Weakly or not expressed in most normal tissues, but mostly inducible in most tissues. Expressed in more than 50% of tumors, either by tumoral, stromal, or endothelial cells (expression in tumor is associated with a worse clinical outcome). Not overexpressed in tumor-draining lymph nodes.
SUBCELLULAR LOCATION
Cytoplasm.
FUNCTION
Indoleamine 2,3-dioxygenase (IDO) is an IFN-γ inducible enzyme that catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine. The gene encoding human IDO maps to chromosome 8p12-p11. IDO, also known as INDO, is an important modulator of immunological responses and protects allogeneic concepti from alloreactive maternal lymphocytes. IDO mediates an interesting inhibitory effect of HeLa cells co-cultured with human PBLs. The ILN-2-induced proliferation response of PBLs is diminished in the presence of HeLa cells while an IDO inhbitor negates this effect. Flow cytometric analysis indicates both mature and immature CD123-positive dentritic cells suppress T cell activity using IDO. IDO-transfected cells co-cultured with T cells reduces T cell proliferation. Additionally, adopted transfer of donor T cells reduces donor T cell numbers in IDO-transgenic mice. The pharmacological or genetic manipulation of IDO may be useful for supressing undesirable T cell response.
CITATIONS
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Evaluation and comparison of the commonly used bioassays of human indoleamine 2, 3-dioxygenase 1 (IDO1) and tryptophan 2, 3-dioxygenase (TDO)
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WB
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Citation
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Song Meiying,et al
Pathogenic Th17 cell-mediated liver fibrosis contributes to resistance to PD-L1 antibody immunotherapy in hepatocellular carcinoma
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IHC
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Mouse
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Citation
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