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NQO1 Recombinant Rabbit Monoclonal Antibody [JF440-1] (ET1702-50)

Price: Regular price $385.00

Volume

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Catalog# ET1702-50

Rabbit Monoclonal to NQO1

Tested for

  • WB

  • IP

  • FC

  • IHC-P

Reacts with

  • Human

  • Mouse

  • Rat

Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
This product has been cited in peer reviewed publications, see list HERE

Safety datasheet

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Specifications

Product Type

Recombinant Rabbit monoclonal primary

Product Name

NQO1 Recombinant Rabbit Monoclonal Antibody [JF440-1] (ET1702-50)

Immunogen

Synthetic peptide within human nqo1 aa 1-49 / 274.

Host

Rabbit

Positive Control

HCT 116 cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, Mouse kidney tissue lysate, Rat kidney tissue lysate, human breast cancer tissue, MCF7.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF440-1

RRID

AB_3070312

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • IP

  • Use at an assay dependent concentration.

  • FC

  • 1:1,000

  • IHC-P

  • 1:1,000

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1ug/ul

Purification

Protein A affinity purified.

Molecular Weight

Predicted band size: 31 kDa

Isotype

IgG

TARGET

UNIPROT #

P15559

PROTEIN NAME

NQO1

SYNONYMS

Azoreductase antibody;Cytochrome b 5 reductase antibody;DHQU antibody;DIA 4 antibody;DIA4 antibody;Diaphorase (NADH/NADPH) (cytochrome b 5 reductase) antibody;Diaphorase (NADH/NADPH) antibody;Diaphorase 4 antibody;Dioxin inducible 1 antibody;DT diaphorase antibody;DT-diaphorase antibody;DTD antibody;Menadione reductase antibody;NAD(P)H dehydrogenase [quinone] 1 antibody;NAD(P)H dehydrogenase quinone 1 antibody;NAD(P)H menadione oxidoreductase 1 dioxin inducible antibody;NAD(P)H: menadione oxidoreductase 1 dioxin inducible 1 antibody;NAD(P)H:menadione oxidoreductase 1 antibody;NAD(P)H:Quinone acceptor oxidoreductase type 1 antibody;NAD(P)H:quinone oxidoreductase 1 antibody;NAD(P)H:quinone oxireductase antibody;NMOR 1 antibody;NMOR I antibody;NMOR1 antibody;NMORI antibody;NQO 1 antibody;NQO1 antibody;NQO1_HUMAN antibody;Phylloquinone reductase antibody;QR 1 antibody;QR1 antibody;Quinone reductase 1 antibody

SEQUENCE SIMILARITIES

Belongs to the NAD(P)H dehydrogenase (quinone) family.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

NAD(P)H:quinone oxidoreductase 1 (NQO1) and NRH:quinone oxidoreductase (NQO2) are flavoproteins that catalyze the metabolic detoxification of quinones and their derivatives to hydroquinones, using either NADH or NADPH as the electron donor. This protects cells against quinone-induced oxidative stress, cytotoxicity, and mutagenicity. Many tumors overexpress NQO1, which is an obligate two-electron reductase that deactivates toxins and activates bioreductive anticancer drugs. NQO1, a 274 amino acid protein, is ubiquitously expressed, but the expression level varies among tissues. NQO1 gene expression is coordinately induced in response to xenobiotics, antioxidants, heavy metals and radiation. The antioxidant response element (ARE) in the NQO1 gene promoter is essential for expression and coordinated induction of NQO1. ARE activation by tert-butylhydroquinone is dependent on PI3-kinase, which lies upstream of Nrf2. Nrf2, c-Jun, Nrf1, Jun-B and Jun-D bind to the ARE and regulate expression and induction of NQO1 gene. Maf-Maf homodimers and possibly Maf-Nrf2 heterodimers play a role in negative regulation of ARE-mediated transcription, but Maf-Nrf1 heterodimers fail to bind with the NQO1 gene ARE and do not repress NQO1 transcription.

CITATIONS

  • Mechanisms of damage to sperm structure in mice on the zinc-deficient diet

  • Mn3O4 Nanozyme Loaded Thermosensitive PDLLA-PEG-PDLLA Hydrogels for the Treatment of Inflammatory Bowel Disease

  • H1N1 influenza virus infection through NRF2-KEAP1-GCLC pathway induces ferroptosis in nasal mucosal epithelial cells

  • Wu Peiyao,et al

    Periodontal pathogen Fusobacterium nucleatum infection accelerates hepatic steatosis in high‐fat diet‐fed ApoE knockout mice by inhibiting Nrf2/Keap1 signaling

  • Synthesis of silver nanoparticles using Eucommia ulmoides extract and their potential biological function in cosmetics

  • Ma Jing,et al

    A Dietary Supplement Jinghuosu Ameliorates Reproductive Damage Induced by Tripterygium Glycosides

  • Cen Qingyan,et al

    CLPs-miR-103a-2-5p inhibits proliferation and promotes cell apoptosis in AML cells by targeting LILRB3 and Nrf2/HO-1 axis, regulating CD8 + T cell response

  • Song Meiying,et al

    Gomisin N rescues cognitive impairment of Alzheimer's disease by targeting GSK3β and activating Nrf2 signaling pathway

  • PD184352 exerts anti-inflammatory and antioxidant effects by promoting activation of the Nrf2/HO-1 axis

  • Yingfei Chen,et al

    Resveratrol inhibits ferroptosis in the lung tissues of heat stroke-induced rats via the Nrf2 pathway

  • Guo, H., Guo, H., Xie, Y., Chen, Y., Lu, C., Yang, Z., Zhu, Y., Ouyang, Y., Zhang, Y., & Wang, X.

    Mo3Se4 nanoparticle with ROS scavenging and multi-enzyme activity for the treatment of DSS-induced colitis in mice

  • Yuan, H., Xu, Y., Luo, Y., Zhang, J. R., Zhu, X. X., & Xiao, J. H.

    Ganoderic acid D prevents oxidative stress-induced senescence by targeting 14-3-3ε to activate CaM/CaMKII/NRF2 signaling pathway in mesenchymal stem cells

  • Yu, Q., Chen, S., Tang, H., Zhang, X., Tao, R., Yan, Z., Shi, J., Guo, W., & Zhang, S.

    Veratric acid alleviates liver ischemia/reperfusion injury by activating the Nrf2 signaling pathway

  • Activation of Nrf2 signaling by 4-octyl itaconate attenuates the cartilaginous endplate degeneration by inhibiting E3 ubiquitin ligase ZNF598

  • Fengjie Huang

    Sinomenine protects against E.coli-induced acute lung injury in mice through Nrf2-NF-κB pathway

  • Liu Y, Lin M, Mu X, et al

    Protective effect of solanesol in glucose-induced hepatocyte injury: Mechanistic insights on oxidative stress and mitochondrial preservation

  • Liu Y, Cui H, Mei C, et al

    Sirtuin4 alleviates severe acute pancreatitis by regulating HIF-1α/HO-1 mediated ferroptosis

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